Proteins such as DNA and RNA are problematic to extract due to temperature sensitivity. The grinding jar of the Retsch CryoMill is continually cooled with liquid nitrogen from the integrated cooling system before and during the grinding process.
From cell disruption to homogenization and pulverization of a great variety of biological samples.
Biological samples exist in all shapes and sizes: hard bones, tough and fibrous plants, tough and viscous sputum, soft muscles, tumor or liver tissue.
Not to mention the millions of cells such as yeast, bacteria or algae, which have to be disrupted for applications such as DNA or RNA isolation or protein extraction.
For research in genomics, transcriptomics or metabolomics, all kinds of biological samples are prepared.
Sample Preparation
Sample preparation is the first step of every analytical process.
Retsch offers a range of mills and grinders for easy and reproducible pulverization of solid sample materials some of which are also suitable for cell disruption and homogenization of biological sample materials.
Cryogenic Grinding
The CryoMill is tailored for cryogenic grinding. The grinding jar is continually cooled with liquid nitrogen from the integrated cooling system before and during the grinding process.
Thus the sample is embrittled and volatile components are preserved. The liquid nitrogen circulates through the system and is continually replenished from an Autofill system in the exact amount which is required to keep the temperature at -196 °C. Powerful impact ball milling results in a perfect grinding efficiency. The Autofill system avoids direct contact with LN2 and makes cryogenic grinding very safe.
